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KMID : 0380619710030020089
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Korean Journal of Food Science and Technology
1971 Volume.3 No. 2 p.89 ~ p.93
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Studies on Milk - clotting Enzyme of Dothiorella ribis
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Abstract
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Microorganisms producing milk-clotting enzyme were isolated from 1,506 strains which were collected from soil on the various places of Korea, and from strains which were already identified. Dothiorella ribis was taken as a good strain producing milk-clotting enzyme. When it is cultured on wheat bran, the optimum experimental conditions for the
production of milk-clotting enzyme were consequently obtained as follows:
1) 30∼35℃ of temperature and 4.0 of pH.
2) 60∼80% of cultivating water to the weight of wheat bran.
3) addition of (NH₄)₂SO₄ as a nitrogen source, NaCl and KH₂PO₄ as an inorganic salt, and 3% of sucrose as a carbon source.
4) four days for a period of cultivation.
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